![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4800022:Design
Designed by: Yao Yao Group: iGEM23_NJTech-China-B (2023-10-07)
MSMEG5739
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1107
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 225
Illegal NgoMIV site found at 820
Illegal NgoMIV site found at 828
Illegal NgoMIV site found at 1240
Illegal NgoMIV site found at 3340
Illegal AgeI site found at 967
Illegal AgeI site found at 1285
Illegal AgeI site found at 2523 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.
Source
MSMEG5739 is from Mycolicibacterium smegmatis MC2 155.